This product is intended for isolation and culture of UU&MH mycoplasma.
The product consists of liquid medium suitable for mycoplasma growth, antibiotic for inhibiting other bacteria growth and positive indicator under strictly sterile condition.
It is simple, rapid in operation without any special equipment, and is easy to read the result without timing required; it is conductive to clinical medication if used together with our mycoplasma identification susceptibility test kit.
UU type: UU mycoplasma liquid culture medium, package insert
MH type: MH mycoplasma liquid culture medium, package insert
Mixed type: UU-MH mixed mycoplasma liquid culture medium, package insert
Main ingredients of liquid culture medium:
Casein peptone, soy peptone, bovine serum, urea, arginine, glucose, phenol red etc
• Stored at -10°C.
• The validity duration is 12 months
• The validity duration is 1 day only once unsealing.
• Male: Collect urethral secretions, prostate secretion, seminal fluid or midstream urine for culture( If it is midstream urine, take 10ml for centrifugal for 10 minutes at 2000r/min, then collect the sediment for inoculation)
• Female: Collect cervix secretion with help of vaginal speculum or amniotic fluid for culture. Urine is not recommended for culture.
Specimen should be tested immediately once getting ready. If not, it should be stored at room temperature but no more than 2h or at 2-8°C no more than 4h.
• Take out the culture medium vial from fridge, thaw and rewarm to room temperature, and record the specimen number.
• Unscrew the vial cap and pull rubber stopper off; then put the cotton swab into the inner wall of the vial closing to culture liquid and squeeze; then discard the swab when specimen submerges into the liquid; and then plug the stopper and screw back the cap; and finally fully shake.
• Inoculate at 35~37°C for 24-48h, then observe the result.
Rewarm Inoculate Culture
• Positive: It indicates positive after inoculating with positive control strain 50ul 10 4 CFU/ml
• Negative: It indicates negative after inoculating with positive control material infected by Candida albicans, Chlamydia pneumoniae, Pseudomonas aeruginosa and Streptococcus.
• For UU tube, observe the result within 24h, it indicates positive if the liquid turns transparently red; otherwise, it indicates negative.
• For MH tube, observe the result after 24h but no later than 48h, it indicates positive if the liquid turns transparently red; otherwise, it indicates negative.
• For UU-MH tube, observe the result no later than 48h, it indicates positive if the liquid turns transparently red; otherwise, it indicates negative.
If liquid turns turbid apparently or turbidly red, repeat the test. If necessary, culture the mycoplasma on solid medium for identification by observing the shape of the colonies with microscopy at lower magnification, when there are typical colonies growing on the medium, it indicates positive.
1. Ideal test result will be likely to obtain following the procedures above completely.
2. The microbiological test mainly depends on the collection of specimen, so a negative result may not indicate that there is no infection of mycoplasama.
3. If red appears in the culture medium, it only indicates that mycoplasma may exist in the medium.
4. For definite clinical diagnosis, it is necessary to appeal to other methods and instruments
1. PH 6.0 ±0.5 (25°C)
2. Repetition Rate: 100%
4. Analytical Specificity: 100%
5. Precision: intra≤5%, inter≤10%
6. Analytical Sensitivity: The lowest value is no less than10 4CFU/ml
If liquid turns turbid apparently or turbidly red, positive result cannot be reported, but some specimen with excessive thin secretion (specially collected from female) may lower the clarity, please differentiate.
Forbidden to use for a woman during menstruation or who suffers from threatened abortion.
Forbidden to use after vaginal medication or vaginal douching.
Put on gloves, gauze mask and protective uniform before the test.
For disposable use only
For in vitro diagnosis use only.
Used culture medium must be autoclaved, then sent to burn in the incinerator together
1. Yinglin Li, The Chinese Medical Encyclopedia [M] . Peking: 2nd edition 1997
2. Yuankang Ye, Yuxing Ni. Mycoplasma hominis and mycoplasma disease, Shanghai Second Medical University press, 1990, 52~58
3. Limb D I. Mycoplasmas of the human genital tract [J], J Med lab Science, 1989, 46:146-148